Hydrogen Sulfide Protects Retinal Pigment Epithelial Cells from Oxidative Stress-Induced Apoptosis and Affects Autophagy

Figure 1

H₂S protects ARPE-19 cells from H₂O₂-induced oxidative damage. (a) MTT assay was performed to detect the cytotoxicity of different concentrations of NaHS in ARPE-19 cells. (b) MTT assay was performed to measure the cytotoxicity of different concentrations of H₂O₂ in ARPE-19 cells. (c) ARPE-19 cells were treated with different concentrations of NaHS and 400 μM H₂O₂. MTT assay was performed to examine the viability of ARPE-19 cells after H₂O₂ exposure for 24 h. (d) Lipid peroxide degradation product MDA was measured using TBA assay after being pretreated with NaHS for 30 min and then exposed to H₂O₂ for 24 h. (e) Cell morphology was examined in a bright field under an inverted fluorescent microscope after being pretreated with NaHS for 30 min and then exposed to H₂O₂ for 24 h. Scale . Values are the . versus to the control group; ^### versus the H₂O₂ treatment alone group.

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