Hydrogen Sulfide Protects Retinal Pigment Epithelial Cells from Oxidative Stress-Induced Apoptosis and Affects Autophagy

Figure 2

H₂S protects ARPE-19 cells against H₂O₂-induced oxidative stress. (a) ARPE-19 cells were pretreated with NaHS for 30 min and exposed to H₂O₂ for 1 h. ROS level was detected using DCFH-DA by flow cytometry. (b) Statistics on the ROS level. (c) Intracellular SOD activity was detected by the assay kit. (d) Intracellular GSH level was measured by the assay kit. (e, f) ELISA detection of secretion of inflammatory factors TNF-α and IL-1β. Cells were pretreated with NaHS for 30 min and then exposed to H₂O₂ for 24 h. Values are the . and versus the control group, ^#, ^##, and ^### versus the H₂O₂ treatment alone group.

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