Hydrogen Sulfide Protects Retinal Pigment Epithelial Cells from Oxidative Stress-Induced Apoptosis and Affects Autophagy

<div>H<sub>2</sub>S decreases H<sub>2</sub>O<sub>2</sub>-induced occurrence of autophagy in ARPE-19 cells. (a) ARPE-19 cells were pretreated with NaHS for 30 min and then treated with H<sub>2</sub>O<sub>2</sub> for 1 h. The protein expression and transform of LC3 I and LC3 II in ARPE-19 cells were analyzed by Western blot. The quantitative analyses of LC3 I/GAPDH, LC3 II/GAPDH, and LC3 II/LC3 I are shown (b–d). (e) Detection of intracellular autophagic vesicles by TEM after being pretreated with NaHS for 30 min and then treated with H<sub>2</sub>O<sub>2</sub> for 24 h. 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: Hydrogen Sulfide Protects Retinal Pigment Epithelial Cells from Oxidative Stress-Induced Apoptosis and Affects Autophagy 