Hydrogen Sulfide Protects Retinal Pigment Epithelial Cells from Oxidative Stress-Induced Apoptosis and Affects Autophagy

Figure 6

Further evidences show that H₂O₂ triggers autophagic flux and that H₂S does not increase autophagic flux in ARPE-19 cells. (a) ARPE-19 cells were treated with 400 μM H₂O₂ for gradient time (0~24 h), and then, cells were collected for Western blot analysis of the autophagy marker proteins LC3 I/II and P62. (b) ARPE-19 cells pretreated with 20 nM Baf A1 (inhibiting the fusion of autophagosomes and lysosomes) for 1 h and then treated with NaHS for 24 h. The LC3 I/II protein expression was also analyzed by Western blot.

(a)

(b)