Blue Light Irradiation Induces Human Keratinocyte Cell Damage via Transient Receptor Potential Vanilloid 1 (TRPV1) Regulation

Figure 7

Blue light-induced production of TNF-α is dependent on TRPV1-mediated signaling. (a) HaCaT cells were irradiated using the indicated intensity of blue light with a wavelength of 470–480 nm and further incubated for 24 h in the presence of capsazepine, SP600125 (JNK inhibitor), or pyrrolidine dithiocarbamate (PDTC). The cells were harvested and subjected to ELISA for examining the TNF-α levels. Data are presented as the of four independent experiments. (b) HaCaT cells were transfected with the NF-κB or AP-1 reporters and the β-galactosidase reporter vector using polyethylenimine, followed by incubation for 16 h. The transfected cells were irradiated using the indicated intensity of blue light with a wavelength of 470–480 nm and further incubated for 24 h in the presence of capsazepine, SP600125, or pyrrolidine dithiocarbamate (PDTC). The cells were harvested and subjected to luciferase reporter assay. Results were obtained from at least three independent experiments, and the values represent the . Statistical significance of differences among the groups was assessed by one-way analysis of variance (ANOVA), followed by Tukey’s multiple comparison test, using the GraphPad Prism 5 software. vs. the control group, ^o vs. the blue light-irradiated control. PDTC: pyrrolidine dithiocarbamate; BL: blue light irradiation.

(a)

(b)