Diazepam and Its Disinfection Byproduct Promote the Early Development of Nervous System in Zebrafish Embryos

Figure 5

Expression level of transcription factors, oct4, sox2, and nanog, were upregulated. (a) The mRNA expression level of (a) oct4 and (b) sox2 were upregulated by DZP and MACB at 10 hpf. (c) The mRNA expression level of nanog was upregulated by MACB at 10 hpf. Data was analyzed by nonparametric, one-way analysis of variance (ANOVA) test, larvae per group, followed with Tukey’s multiple comparisons test, expressed as . ; ; ; NS: not significant. (d) Diagram showing how DZP and MACB affect early proliferation and differentiation and nervous activity.

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