Polydatin Attenuates 14.1 MeV Neutron-Induced Injuries via Regulating the Apoptosis and Antioxidative Pathways and Improving the Hematopoiesis of Mice

Figure 5

Mouse splenic and lung apoptosis changes after IR/sham combined with/without PD administration. (a) Representative dot plots chosen randomly from each treatment group. The axis indicates FITC fluorescence intensity, and the axis represents PI fluorescence intensity (as the data were generated via the Beckman Coulter flow cytometry (CytoFLEX) and analyzed by the CytoExpert software, of which the channel of PC5.5 covers the wavelength of PI dye, and the channel name is PC5.5). The events of each quadrants were discriminated with different colors with the corresponding percentage showing in each corner. (b) The calculated statistical data from three independent identical experiments. Data are , . and vs. the DMSO group; ^## vs. the IR DMSO group. (c) Immunodetection of mouse lung tissue from different treatment groups. Two samples were randomly selected from each group and underwent the WB analysis. For each well, 15 μg protein were loaded here.

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