The Effects of Alpha-Linolenic Acid on the Secretory Activity of Astrocytes and β Amyloid-Associated Neurodegeneration in Differentiated SH-SY5Y Cells: Alpha-Linolenic Acid Protects the SH-SY5Y cells against β Amyloid Toxicity

Figure 1

Scheme of the experimental procedures. The first step of the research involved the preparation of astrocyte-conditioned medium (CM) and Alpha-linolenic acid-preactivated CM (ALA-CM). The Normal Human Astrocytes (NHA) cells were allowed to grow until 60-70% confluence. Then, NHA cells were grown in the medium containing NHA-Medium and neurobasal medium (the phase II differentiation medium DM II) (1 : 1). After 24 h, the medium was replaced with DM II medium alone or with 10 nM ALA for the next 24 h incubation to obtain CM and ALA-preactivated-CM. The second step included the evaluation of the neuroprotective effect of CM and ALA-CM on Amyloid β_1-42- (Aβ_1-42-) induced neurodegeneration of differentiated SH-SY5Y cells. On the day 6th, the SH-SY5Y cells (differentiated) were pretreated for 1 h with CM or ALA-CM before the addition of 5 μM Aβ_1-42.