Research Article
The Effects of Alpha-Linolenic Acid on the Secretory Activity of Astrocytes and β Amyloid-Associated Neurodegeneration in Differentiated SH-SY5Y Cells: Alpha-Linolenic Acid Protects the SH-SY5Y cells against β Amyloid Toxicity
Figure 5
CM and ALA-CM reversed the effects of Amyloid β1-42 (Aβ1-42) treatment on differentiated SH-SY5Y cell viability. The results showed that the CM and ALA-CM pretreatment significantly increased the viability of differentiated SH-SY5Y cells and restored Aβ1-42-induced reduction of the cell viability (a). Insulin Degrading Enzyme (IDE) treatment of CM and ALA-CM reduced this effect. On the day 6th, the SH-SY5Y cells (differentiated) were pretreated for 1 h with CM or ALA-CM before the addition of 5 μM Aβ1-42 for the next 24 h. The SH-SY5Y cells were also exposed to cotreatment of CM and ALA-CM with IDE to check whether insulin and IGF-I presence in CM and ALA-CM was responsible for the neuroprotective effect. Positive controls were the SH-SY5Y cells treated with insulin (c) and carbonyl-cyano-m-chlorophenylhydrazone-CCCP (10 μM). The obtained results are presented as a percentage of the control value. One-way ANOVA test for viability followed by Tukey’s multiple comparisons was used to analyse data. Statistical differences between the treated cells and untreated control cells are indicated by asterisks ( for ; for ; for ; # versus the control group). Results are of three independent experiments. Cell morphology was observed under a microscope (b). Scale bar is 20 μm.
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