Oxidative Medicine and Cellular Longevity

Research Article

The Effects of Alpha-Linolenic Acid on the Secretory Activity of Astrocytes and β Amyloid-Associated Neurodegeneration in Differentiated SH-SY5Y Cells: Alpha-Linolenic Acid Protects the SH-SY5Y cells against β Amyloid Toxicity

Table 1

Primers and their sequences used to identify genes in the quantitative real-time reverse-transcription-polymerase chain reaction (RT-qPCR).
Gene nameForward primer sequencesReverse primer sequences
Mitophagy genes
 PINK1GGACGCTGTTCCTCGTTAATCTGCGATCACCAGCCA
 PARKINCCCACCTCTGACAAGGAAACATCGTGAACAAACTGCCGATCA
Autophagy genes
 ATG5ATGATAATGGCAGATGACAAGGTCAGTCACTCGGTGCAGG
 LC3βAAAGGAGGACATTTGAGCAGAATGTCTCCTGGGAAGCGTA
Mitochondrial biogenesis genes
 TFAMGTGGGAGCTTCTCACTCTGGTAGGGCTTTTTCTCCTGCAA
 PGC1αCACCAGCCAACACTCAGCTAGTGTGAGGAGGGTCATCGT
Mitochondrial dynamics genes
 MFN2AATCTGAGGCGACTGGTGACTCCTCCTGTTCGACAGTCA
 OPA1GGCCAGCAAGATTAGCTACGACAATGTCAGGCACAATCCA
 DRP1GGCAACTGGAGAGGAATGCCTTTTTGTGGACT
Synaptic genes
 PSD95CTTCATCCTTGCTGGGGGTCTTGCGGAGGTCAACACCATT
 SynaptophysinCTGCGTTAAAGGGGGCACTAACAGCCACGGTGACAAAGAA
Reference gene
 GAPDHGAAGGTGAAGGTCGGAGTGAAGATGGTGATGGGATTTC