Formation and Detection of Highly Oxidized Hemoglobin Forms in Biological Fluids during Hemolytic Conditions

Figure 5

Time-dependent accumulation of covalently crosslinked Hb multimers in biological samples under hemolytic conditions. (a–d) Plasma and urine samples were collected from C57BL/6 mice at different time points (/group) following PHZ injection. (a, c) Representative western blots and (b, d) densitometric analysis of western blots are presented. The bar graph shows from 3 independent experiments. (e, f) CSF samples were obtained from preterm infants diagnosed with grade III IVH at different time intervals after the onset of IVH (/group). (e) Representative western blot is shown. Densitometric analysis was performed and percentages of Hb monomers, dimers, and tetramers as a percent of total Hb were calculated. values were calculated using one-way ANOVA followed by Tukey’s multiple comparison analysis. , , , .

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