Resveratrol Mitigates Sevoflurane-Induced Neurotoxicity by the SIRT1-Dependent Regulation of BDNF Expression in Developing Mice

Figure 4

Resveratrol prevented sevoflurane-induced decreases in SIRT1 and BDNF ex vivo and in vitro. (a) Neonatal mice were pretreated with 50 mg/kg, 100 mg/kg, and 150 mg/kg resveratrol from P3 to P8 and exposed to sevoflurane from P6 to P8. Western blotting was performed to determine the minimum effective dose of resveratrol. (b) Primary hippocampal neurons were pretreated with 10 μM, 20 μM, and 40 μM resveratrol 24 h before exposure to sevoflurane. Western blotting was used to determine the minimum effective concentration of resveratrol. (c, d) RT-PCR results showing SIRT1 and BDNF mRNA expression in the developing mouse hippocampus between groups (control group vs. sevoflurane group) and treatments (DMSO or resveratrol), respectively. (e) Representative western blot bands of SIRT1 and BDNF in the developing mouse hippocampus between groups (control group vs. sevoflurane group) and treatments (DMSO or resveratrol). (f) Representative western blot bands of SIRT1 and BDNF in hippocampal neurons between groups (control group vs. sevoflurane group) and treatments (DMSO or resveratrol). (g, i) Densitometry quantification of SIRT1 and BDNF ex vivo, respectively. (h, j) Densitometry quantification of SIRT1 and BDNF protein expression in vitro, respectively. Data are shown as ( per group). , , and .

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