Resveratrol Mitigates Sevoflurane-Induced Neurotoxicity by the SIRT1-Dependent Regulation of BDNF Expression in Developing Mice

Figure 5

Resveratrol reversed the change in the expression of MeCP2 and CREB induced by sevoflurane exposure ex vivo and in vitro. (a) Co-IP results for SIRT1, AcMeCP2, and CREB. (b) Representative western blot bands of AcMeCP2, total MeCP2, p-CREB, and total CREB in the developing mouse hippocampus between groups (control group vs. sevoflurane group) and treatments (DMSO or resveratrol). (c) Representative western blot bands of AcMeCP2, total MeCP2, p-CREB, and total CREB in primary hippocampal neurons between groups (control group vs. sevoflurane group) and treatments (DMSO or resveratrol). (d, f, h) Densitometry quantification of AcMeCP2, total MeCP2, p-CREB, and total CREB expression ex vivo, respectively. (e, g, i) Densitometry quantification of AcMeCP2, total MeCP2, p-CREB, and total CREB expression in vitro, respectively. Data are shown as ( per group). , .

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