Role of Mesencephalic Astrocyte-Derived Neurotrophic Factor in Alcohol-Induced Liver Injury

Figure 3

Hepatocyte MANF deletion activates inflammatory cells in alcohol-induced liver injury. (a) MPO⁺ neutrophils were detected by the immunohistochemical assay in WT and MANF^ΔHep mice fed with ethanol (EtOH). Scale . (b) The quantitative data of panel (a). (c) The mRNA level of Ly6G was detected by quantitative real-time PCR. Values represent (-8). , , and . (d) Liver tissues were subjected to immunostaining with an anti-CD68 antibody. Scale . (e) The mRNA level of CD68 was detected by quantitative real-time PCR. The values were expressed as (–8). , .

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