Colchicine Alleviates Cholesterol Crystal-Induced Endothelial Cell Pyroptosis through Activating AMPK/SIRT1 Pathway

Figure 5

Colchicine reverses the downregulation of various antioxidant enzymes and promotes the activation of AMPK-SIRT1 signaling in endothelial cells. HUVECs were treated for 24 h with different doses of colchicine (0–10 nM) in the presence of cholesterol crystal (0.5 mg/ml) or were left untreated (control). (a) Western blotting was dedicated to examine the protein expression levels of SIRT1, p-AMPKα, AMPKα, HO-1, SOD-2, and SOD-1. (b–f) Quantitative analysis of AMPK/SIRT1 pathway-associated protein expression. Data was expressed as the of three separate experiments. , vs. the control group. ^#, ^##, and ^### vs. the cholesterol crystal group.

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