Research Article
Dexmedetomidine Postconditioning Alleviates Hypoxia/Reoxygenation Injury in Senescent Myocardial Cells by Regulating lncRNA H19 and m6A Modification
Figure 2
Dexmedetomidine (Dex) hypoxia postconditioning attenuated oxidative stress damage and hypoxia/reoxygenation-induced apoptosis in senescent cardiomyocytes. (a) Senescent cardiomyocytes were exposed to 0, 500 nM, 1 μM, or 2 μM Dex for 6 h after hypoxia for 6 h. Cells were stained by DCFH-DA to measure levels of reactive oxygen species. Mean fluorescence intensity of DCF was detected by flow cytometry. (b) A JC-1 probe was used to observe and detect fluorescence ratios of red and green in each group. (c) FITC/PI staining was used to detect ratios of apoptotic cells in each group by flow cytometry. (d) Viability of senescent H9c2 cells treated with different concentrations of Dex postconditioning was measured by CCK-8. (e) Expression of PUMA mRNA in each group was detected by RT-PCR. (f) Ratios of Bcl-2/Bax were evaluated by western blots. , ; vs. the H/R group.
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