Dexmedetomidine Postconditioning Alleviates Hypoxia/Reoxygenation Injury in Senescent Myocardial Cells by Regulating lncRNA H19 and m<sup>6</sup>A Modification

<div>Dexmedetomidine (Dex) postconditioning alleviated hypoxia/reoxygenation injury by upregulating H19. (a) H19 expression after exposure to different concentrations of Dex and hypoxia postconditioning was detected by RT-PCR (<span class="nowrap"><svg height="9.10327pt" id="M22" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -8.75334 58.1969 9.10327" width="58.1969pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.595,0)"><path d="M380 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109 431 165 431 225C431 308 366 395 238 395C212 395 165 379 127 364L153 550Z"></path></g></svg>vs. the H/R group). (b) After 24 h, normal DMEM culture was replaced with 150 <i>μ</i>M H<sub>2</sub>O<sub>2</sub> for 4 h, and H9c2 cells were transfected with siH19 or a negative control (NC) siRNA. After hypoxia for 6 h, 1 <i>μ</i>M Dex was added during reoxygenation for 6 h. Expression of H19 was detected by RT-PCR. (c) Cell viability was evaluated using a CCK-8 assay. (d) Ratios of Bcl-2/Bax protein expression were measured by western blotting. <span class="nowrap"><svg height="9.10327pt" id="M25" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -8.75334 58.8493 9.10327" width="58.8493pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M861 0V28C774 35 771 41 768 147L759 509C756 612 762 614 851 622V650H681L449 149L221 650H57V622C148 613 153 609 144 479L130 271C123 166 117 123 111 88C104 46 85 34 26 28V0H259V28C192 35 169 42 167 90C166 130 166 173 170 256L185 541H187L411 7H431L675 555H679L683 147C683 41 680 35 598 28V0H861Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.244,0)"><path d="M380 106C343 72 306 56 265 56C195 56 116 112 115 248C235 252 361 262 377 265C396 269 400 277 400 297C400 374 333 449 250 449H249C198 449 144 421 103 376S37 269 37 201C37 88 109 -12 232 -12C263 -12 332 6 395 84L380 106ZM225 412C281 412 315 364 314 312C314 297 308 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Oxidative Medicine and Cellular Longevity

fig3

Figure 3

Figure 3: Dexmedetomidine Postconditioning Alleviates Hypoxia/Reoxygenation Injury in Senescent Myocardial Cells by Regulating lncRNA H19 and m<sup>6</sup>A Modification 

Figure 3 | Dexmedetomidine Postconditioning Alleviates Hypoxia/Reoxygenation Injury in Senescent Myocardial Cells by Regulating lncRNA H19 and m6A Modification 