Dexmedetomidine Postconditioning Alleviates Hypoxia/Reoxygenation Injury in Senescent Myocardial Cells by Regulating lncRNA H19 and m⁶A Modification

Figure 6

Dex postconditioning attenuated hypoxia/reoxygenation- (H/R-) induced increases of m⁶A levels and downregulated ALKBH5 in aged H9c2 cells. (a) Colorimetry was used to detect total m⁶A modification levels of RNA in aged myocardial cells after H/R and Dex postconditioning (, , ). (b) mRNA levels of m⁶A modification-associated genes were measured by qRT-PCR (, , ). (c) mRNA levels of ALKBH5 following H/R and H/R+Dex (, , ). (d) ALKBH5 expression of cells exposed to H/R and H/R+Dex was measured by western blot (, , ). Abbreviation: METTL3: methyltransferase like 3; METTL14: methyltransferase like 14; ALKBH5: alkB homolog 5; WTAP: Wilms tumor 1-associated protein; FTO: fat mass and obesity-associated protein; YTHDF1: YT521-B homology m6A-binding protein 1; YTHDC1: YT521-B homology domain-containing 1; HNRNPC: heterogeneous nuclear ribonucleoprotein C.

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