Dexmedetomidine Postconditioning Alleviates Hypoxia/Reoxygenation Injury in Senescent Myocardial Cells by Regulating lncRNA H19 and m⁶A Modification

Figure 7

H19 undergoes m⁶A modification via regulation of ALKBH5. (a) ALKBH5 protein expression was detected 48 h after knockdown by siRNA transfection using western blots. (b) Expression of H19 after transfection of siALKBH5 or NC was detected by qRT-PCR (, , ). (c) ALKBH5 protein expression was measured by western blot after transfection with a vector containing ALKBH5 or empty vector. (d) H19 expression after transfection with a vector encoding ALKBH5 or empty vector was detected by qRT-PCR (, , ). (e) H9c2 cells were treated by transfection of siH19 to reduce expression of H19. The efficiency of transfection was detected by qRT-PCR after 48 h (, , ). (f) ALKBH5 mRNAs were detected by qRT-PCR (, , ). (g) Protein expression levels of ALKBH5 were measured by western blot. (h) H19 m⁶A methylation levels were measured after ALKBH5 knockdown (, , ).

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