Heme Oxygenase-1-Mediated Autophagy Protects against Oxidative Damage in Rat Nucleus Pulposus-Derived Mesenchymal Stem Cells

Figure 1

Cell viability, apoptosis, and ROS production of NPMSCs with H₂O₂ treatment. (a) Cell viability of NPMSCs treated with different concentrations (0, 40, 80, 120, and 160 μM) of H₂O₂ for 24 hours. (b) Cell viability of NPMSCs treated with 120 μM of H₂O₂ for different times (0, 1, 12, and 24 hours). (c) TUNEL staining of NPMSCs ( μm). (d) Representative images of cell apoptosis detected by flow cytometry after Annexin V/PI dual staining. (e) Summary data showing the apoptosis rate in different groups. The apoptotic cells were stained with Annexin V+/PI- and Annexin V+/PI+. (f) ROS production evaluated by fluorescence staining and flow cytometry ( μm). (g) Summary data showing the level of ROS in different groups. NS means no significant difference. The data are expressed as the from three independent experiments (, vs. 0 μM of H₂O₂).

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