Research Article
Heme Oxygenase-1-Mediated Autophagy Protects against Oxidative Damage in Rat Nucleus Pulposus-Derived Mesenchymal Stem Cells
Figure 2
The expression level of HO-1 in NPMSCs with different treatments. (a) The typical western blot bands of HO-1 in NPMSCs treated with H2O2 for different times. (b) Summary data showing protein levels of HO-1. (c) The typical western blot bands of HO-1 in NPMSCs transfected with negative control siRNA (NC) and three different HO-1-siRNA. (d) Summary data showing protein levels of HO-1. (e) The typical western blot bands of HO-1 in NPMSCs pretreated with negative control siRNA (NC), HO-1-siRNA, or CoPP and then exposed to H2O2 for 1 hour. (f) Summary data showing protein levels of HO-1. (g) The typical western blot bands of HO-1 in NPMSCs pretreated with negative control siRNA (NC), HO-1-siRNA, or CoPP and then exposed to H2O2 for 24 hours. (h) Summary data showing protein levels of HO-1. NS means no significant difference. The data are expressed as the from three independent experiments (, vs. 0 μM of H2O2 group, NC group, or NC+H2O2 treatment group; # vs. si-HO-1+H2O2 treatment group).
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