Research Article
Heme Oxygenase-1-Mediated Autophagy Protects against Oxidative Damage in Rat Nucleus Pulposus-Derived Mesenchymal Stem Cells
Figure 5
HO-1 regulated the expressions of autophagy-related proteins in NPMSCs with different treatments. (a) The typical western blot bands of LC3 and Beclin-1 in NPMSCs pretreated with negative control siRNA (NC), HO-1-siRNA, or CoPPand then exposed to H2O2 for 1 hour. (b, c) Summary data showing protein levels of LC3 II and Beclin-1. (d) The typical western blot bands of LC3 and Beclin-1 in NPMSCs pretreated with negative control siRNA (NC), HO-1-siRNA, or CoPP and then exposed to H2O2 for 24 hours. (e, f) Ssummary data showing protein levels of LC3 II and Beclin-1. NS means no significant difference. The data are expressed as the from three independent experiments (, vs. NC+H2O2 treatment group; #, ##, and ### vs. si-HO-1+H2O2 treatment group).
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |