Heme Oxygenase-1-Mediated Autophagy Protects against Oxidative Damage in Rat Nucleus Pulposus-Derived Mesenchymal Stem Cells

Figure 6

Cell viability, apoptosis, and ROS production of NPMSCs pretreated with negative control siRNA (NC), HO-1-siRNA, or RAP and then exposed to H₂O₂. (a) Cell viability of NPMSCs pretreated with negative control siRNA (NC), HO-1-siRNA, or RAP and then exposed to H₂O₂ for 1 hour. (b) Cell viability of NPMSCs pretreated with negative control siRNA (NC), HO-1-siRNA, or RAP and then exposed to H₂O₂ for 24 hours. (c) Representative images of cell apoptosis detected by flow cytometry. NPMSCs were treated as in (a). (d) Representative images of cell apoptosis detected by flow cytometry. NPMSCs were treated as in (b). (e, f) Summary data showing the apoptosis rate in different groups. The apoptotic cells were stained with Annexin V+/PI- and Annexin V+/PI+. (g) Representative images of ROS production evaluated by flow cytometry. NPMSCs were treated as in (a). (h) Representative images of ROS production evaluated by flow cytometry. NPMSCs were treated as in (b). (i, j) Summary data showing the level of ROS in different groups. The data are expressed as the from three independent experiments (, , and vs. NC+H₂O₂ treatment group; ^#, ^##, and ^### vs. si-HO-1+H₂O₂ treatment group).

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