Mitochondrial-Protective Effects of R-Phenibut after Experimental Traumatic Brain Injury

Figure 4

Cresyl violet (Nissl) and IL-1β immunofluorescence staining 7 days post-TBI. (a) Cresyl violet-stained sections of the mouse neocortex ipsilateral to the injury site. R-Phenibut treatment at doses of 10 mg/kg and 50 mg/kg reduced the number of N-DNs. . (b) IL-1β expression based on immunofluorescence staining in the mouse neocortex ipsilateral to the injury site. R-Phenibut treatment at doses of 10 mg/kg and 50 mg/kg reduced the number of IL-1β-positive cells. . (c) Schematic illustration of the brain region indicated in the filled area, which was selected for the quantitative analysis of cell injury. (d) Quantitative assessment of N-DNs in the ipsilateral cortex at postinjury day 7. Data are expressed as the ( for the R-phenibut 50 mg/kg group and n = 6 for the sham, control, and R-phenibut 10 mg/kg groups). (e) Quantitative assessment of IL-1β-positive cells in the ipsilateral cortex at postinjury day 7. Data are expressed as the ( for the control group and for the sham, R-phenibut 10 mg/kg, and 50 mg/kg groups). ^#Indicates a significant difference compared to the sham-operated group; indicates a significant difference compared to the control group (one-way ANOVA followed by Tukey’s multiple comparison test; ).

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