Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 3

Research Article

Targeting the Nrf2/ARE Signalling Pathway to Mitigate Isoproterenol-Induced Cardiac Hypertrophy: Plausible Role of Hesperetin in Redox Homeostasis

Figure 3

mRNA levels, protein expression, and activity of GCLC, NQO1, and HO-1. (a) Gene expression levels of GCLC, NQO1, and HO-1 (left panel); L1: marker; L2: control; L3: isoproterenol; L4: isoproterenol+hesperetin; L5: hesperetin. Relative mRNA levels—band intensity normalized with β-actin (right panel). (b) Immunoblot analysis of antioxidant protein expression in the heart. Actin served as a loading control. The protein levels were normalized to the actin level. (c) Bar plots showing the activity of GCLC, NQO1, and HO-1. Results are shown as the mean ± SEM of three separate experiments. Statistical significance (, , and ) was calculated by Student-Newman-Keuls and Tukey post hoc tests.

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