Blueberry Extracts as a Novel Approach to Prevent Ozone-Induced Cutaneous Inflammasome Activation

Figure 5

Blueberry (BB) extract prevents O₃-induced inflammasome oligomerization in HaCaT cells. (a) Immunofluorescence staining of ASC (green), NLRP1 (red), DAPI (blue), and merge in HaCaT cells pretreated with 10 μg/ml of BB for 24 h then exposed to 0.5 ppm of O₃ for 1 h at 0, 3, and 6 h postexposure (40x magnification). On the right, ASC speck formation was quantified using ImageJ. (b) ASC oligomers, dimers, monomers, and input protein levels in keratinocytes at 0, 1, and 3 h after O₃ exposure (1 h, 0.5 ppm). On the right, depiction of ASC oligomers and dimer quantification using ImageJ. Data are the results of three independent experiments. air vs. O₃, ^§ O₃ vs. BBO₃ by two-way ANOVA.

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