Critical Evaluation of the Interaction of Reactive Oxygen and Nitrogen Species with Blood to Inform the Clinical Translation of Nonthermal Plasma Therapy

Figure 2

Quantification of persistent RNS and H₂O₂ in three aqueous solutions: PBS, blood plasma (BP), and processed whole blood. NTP was generated in direct contact with the target liquid (direct NTP) or used to enrich PBS, which was then transferred to the target liquid (indirect NTP). Following NTP treatment, RNS and H₂O₂ analysis was performed immediately (0 s) or after a delay of 30 to 300 seconds to assess the stability of these species in (a, b) PBS, (c, d) BP, and (e, f) processed whole blood. Data are represented as . ROS/RNS concentration following NTP treatment at all time points was compared to untreated and statistical significance was determined using the generalized linear mixed model (). ; ; ^#.

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