Effects of the Insulted Neuronal Cells-Derived Extracellular Vesicles on the Survival of Umbilical Cord-Derived Mesenchymal Stem Cells following Cerebral Ischemia/Reperfusion Injury
Apoptosis of UC-MSCs cultured with conditioned medium from injured N2A cells. UC-MSCs were cultured with conditioned medium from N2A cells under non-OGD/R condition or OGD/R24H insult. (a–b) Apoptosis of UC-MSCs as evaluated by flow cytometry with Annexin-V/PI staining assay. (c) Apoptosis of UC-MSCs as evaluated by LDH leakage assay. (d) Cell viability of UC-MSCs as evaluated by MTT assay. (e) Expression and quantitative data of cleaved-caspase3, cleaved-caspase9, and cytochrome C as evaluated by western blotting. (f–g) Apoptosis of UC-MSCs as evaluated by TUNEL immunofluorescence. . All data are presented as the ().;, R24H-N2A-CMs group compared with N2A-CMs group.
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