Effects of the Insulted Neuronal Cells-Derived Extracellular Vesicles on the Survival of Umbilical Cord-Derived Mesenchymal Stem Cells following Cerebral Ischemia/Reperfusion Injury

Figure 4

Effects of EVs derived from N2A cells on apoptosis of UC-MSCs upon non-OGD/R condition. UC-MSCs were cultured with two various concentration EVs under non-OGD/R condition for 24 hours. (a–b) Apoptosis of UC-MSCs as evaluated by flow cytometry with Annexin-V/PI staining assay. (c) Apoptosis of UC-MSCs as evaluated by LDH leakage assay. (d) Cell viability of UC-MSCs as evaluated by MTT assay. (e–f) Expression and quantitative data of cleaved-caspase3, cleaved-caspase9, and cytochrome C as evaluated by western blotting. EVs-1: represented as higher concentration EVs recorded by NTA assay. EVs-2: represented as lower concentration EVs recorded by NTA assay. All data are presented as the (). ; , compared with the control group.

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