Effects of the Insulted Neuronal Cells-Derived Extracellular Vesicles on the Survival of Umbilical Cord-Derived Mesenchymal Stem Cells following Cerebral Ischemia/Reperfusion Injury

Figure 6

Effect of EVs derived from N2A cells on oxidative stress level of UC-MSCs. UC-MSCs were cultured with two various concentration EVs under non-OGD/R condition and OGD/R24H insult, respectively. (a) Levels of ROS as determined by dihydroethidium-DCFH-DA (DHE) under non-OGD/R condition. (b) Levels of as determined by firefly luciferase assay under non-OGD/R condition. (c) Levels of SOD as determined by WST-8 assay under non-OGD/R condition. (d) Levels of T-AOC as determined by ABTS assay under non-OGD/R condition. (e) Levels of ROS as determined by dihydroethidium-DCFH-DA assay upon OGD/R24H insult. (f) Levels of ATP as detected by firefly luciferase assay upon OGD/R24H insult. (g) Levels of SOD as determined by WST-8 assay upon OGD/R24H insult. (h) Levels of T-AOC as determined by ABTS assay upon OGD/R24H insult. EVs-1: represented as higher concentration EVs recorded by NTA assay. EVs-2: represented as lower concentration EVs recorded by NTA assay. All data are presented as the (). ; , compared with control group or normal culture medium group.