Neuroprotection Effect of Astragaloside IV from 2-DG-Induced Endoplasmic Reticulum Stress

Figure 1

Effects of 2-DG on ERS and the mPTP opening. (a) Western blot images of GRP 78 and GRP 94 expression in PC12 cells treated with vehicle control and 25, 50, 100, 150, 200, and 500 μM 2-DG. (b) Confocal fluorescence images of cells incubating TMRE for 20 minutes in PC12 cells treated with 0, 25, 50, 100, 150, 200, and 500 μM 2-DG. (c, d) Quantification of (a). Each protein expression level was normalized with GAPDH. (e) Quantification of (b). Data are of 6 independent experiments. compared to vehicle control. Scale bar, 50 μm.

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