Research Article
Neuroprotection Effect of Astragaloside IV from 2-DG-Induced Endoplasmic Reticulum Stress
Figure 3
Effect of astragaloside IV on PC12 cell survival from ERS. (a) Representative Western blot images of Cleaved-Caspase-3 in PC12 cells treated with 2-DG in different times. (b) Representative Western blot images of Cleaved-Caspase-3 in PC12 cells treated with 2-DG and astragaloside IV. (c, d) Confocal fluorescence images and flow cytometry of Annexin V-FITC Apoptosis Detection kit in PC12 cells treated with 2-DG and astragaloside IV. Cells were preloaded with Annexin V and PI for 20 minutes before other treatments. PC12 cell culture was placed on the temperature-controlled stage and treated with astragaloside IV for 20 minutes and 2-DG for 6 h. Data are of 6 independent experiments. compared to control; # compared to 2-DG. Scale bar, 50 μm.
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