LCZ696 Ameliorates Oxidative Stress and Pressure Overload-Induced Pathological Cardiac Remodeling by Regulating the Sirt3/MnSOD Pathway

Figure 3

LCZ696 treatment attenuated TAC-induced cardiac fibrosis. (a) Representative images of Masson trichrome staining in different treatment groups. (b) Representative images of Picric Sirius red (PSR) staining of cardiac interstitial and perivascular regions in different treatment groups. (c) Representative immunohistochemical staining of α-SMA for myofibroblasts. (d) Quantification of the percentage of left ventricular fibrosis area from (b) ( mice per group). (e) mRNA levels of collagen I, collagen III, TGF-β, and CTGF in the indicated groups ( mice per group). The results are normalized against GAPDH and converted to fold change relative to the sham+vehicle group ( mice per group). Data are presented as . vs. the sham+vehicle group, ^# vs. the TAC+vehicle group.

(a)

(b)

(c)

(d)

(e)