Research Article

LCZ696 Ameliorates Oxidative Stress and Pressure Overload-Induced Pathological Cardiac Remodeling by Regulating the Sirt3/MnSOD Pathway

Figure 8

Sirt3 knockdown abolished the antihypertrophic effect of LCZ696 in PE-induced cardiomyocyte hypertrophy. (a) Immunofluorescence staining of α-actinin in primary neonatal rat cardiomyocytes (NRCMs) in the indicated groups. (b) The cell surface area in the indicated groups ( cells per experimental group), vs. the si-NC+vehicle group, # vs. the si-NC+LCZ696 treatment group. (c, d) Western blot analysis and quantitative analysis of Bcl-2 and Bax protein levels from different treatment groups. (e) Representative images of DCFH-DA staining in NRCMs. (f) Representative images of MitoSOX staining in NRCMs. (g, h) Western blot analysis of p-AMPK and AMPK protein levels in different treatment NRCMs and quantitative analysis of the ratio of p-AMPK/AMPK, . vs. control group, # vs. PE treatment group.