PI3K/Akt and ERK1/2 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury
Effects of quercetin on the survival of P19 neurons in the presence of excess copper. For the analysis of neuronal survival, P19 neurons (/well) were exposed to the indicated concentrations of CuSO4 and quercetin in a 96-well plate for 24 h, and for 3 h with MTT afterwards. Precipitated formazan crystals were dissolved with DMSO, and the absorbance was recorded at 570 nm. In moderately injured neurons exposed to 0.5 mM CuSO4, 150 μM quercetin improved neuronal survival (a). Treatment with 1 mM CuSO4 induced more severe neuronal death. The presence of 30 μM quercetin further exacerbated the cytotoxic effects of copper, whereas a 150 μM concentration was without effect (b). Data represent the from four independent experiments performed in quadruplets. vs. cont (0 group); a and b vs. the copper-treated group (one-way ANOVA followed by Tukey’s test: (a) ,; (b) ,).