Folic Acid Protects Melanocytes from Oxidative Stress via Activation of Nrf2 and Inhibition of HMGB1

<div>FA protected PIG1 cells from H<sub>2</sub>O<sub>2</sub>-induced apoptosis. PIG1 cells were exposed to 800 <i>μ</i>M H<sub>2</sub>O<sub>2</sub> and FA at indicated concentrations for 48 h simultaneously. (a, b) The percentage of apoptotic cells was determined by flow cytometry assay. Bar graphs represent the mean values of flow cytometry data. (c) Effects of FA on H<sub>2</sub>O<sub>2</sub>-induced expressions of Bcl-2, Bax, Casepase-3, and cleaved Caspase-3 were determined via Western blot. <i>β</i>-Actin was detected as loading control. Bar graphs represent the quantification of Bcl-2/Bax ratio and cleaved/full length Caspase-3 ratio via gray intensity analysis across three replicate experiments. 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Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: Folic Acid Protects Melanocytes from Oxidative Stress via Activation of Nrf2 and Inhibition of HMGB1 