Folic Acid Protects Melanocytes from Oxidative Stress via Activation of Nrf2 and Inhibition of HMGB1

Figure 4

Nrf2 knockdown abolished the protective effects of FA on PIG1 cells against oxidative damages induced by H₂O₂. PIG1 cells were transfected with the shRNA against Nrf2 or control shRNA for 24 h and then treated with H₂O₂ and FA at indicated concentrations for 48 h. (a) The interference efficiency of Nrf2 shRNA was evaluated via Western blot. β-Actin was detected as loading control. (b, c) Intracellular ROS level was determined by flow cytometry assay. Bar graphs represent the mean values of the fluorescence intensity of ROS level. (d, e) The percentage of apoptotic cells was determined by flow cytometry assay. Bar graphs represent the mean values of flow cytometry data. (f) The expressions of Nrf2, p-Nrf2, SOD2, and HO-1 were determined via Western blot. β-Actin was detected as loading control. , , ; ns: not significant.

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