XIST Inhibition Attenuates Calcium Oxalate Nephrocalcinosis-Induced Renal Inflammation and Oxidative Injury via the miR-223/NLRP3 Pathway

Figure 3

miR-223-3p directly bound to the 3-UTR of XIST to suppress its expression. (a) Diagrammatic sketch of the WT and mutated XIST 3-UTR targeting miR-223-3p. (b) qRT-PCR analysis was performed to determine the level of miR-223-3p in HK-2 cells. (c) Polarizing microscopy was performed to verify the crystal deposition in the CaOx nephrocalcinosis model. Fluorescence in situ hybridization was performed to examine the expression of XIST and miR-223-3p in the CaOx nephrocalcinosis model under different treatments. (d) The relationship between XIST and miR-223-3p was determined by Pearson analysis. The interaction of the 3-UTR of XIST and the miR-223-3p mimics (e) or inhibitor (f) was verified via the dual-luciferase reporter gene assay. (g) The XIST levels in HK-2 cells were quantified by qRT-PCR. The data are presented as the of three independent experiments. ; , as determined by one-way ANOVA (b, e–g) and Pearson’s correlation test (d).

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