XIST Inhibition Attenuates Calcium Oxalate Nephrocalcinosis-Induced Renal Inflammation and Oxidative Injury via the miR-223/NLRP3 Pathway

Figure 4

miR-223-3p inhibited the NLRP3 expression by directly binding to its 3-UTR. (a) Diagrammatic sketch of the WT and mutated NLRP3 3-UTR targeting miR-332-3p. (b) Pearson correlation analysis of NLRP3 and miR-223-3p was performed. A dual-luciferase reporter assay was performed to examine the interaction between the 3-UTR of NLRP3 and the miR-223-3p mimics (c) or inhibitor (d). The expression levels of NLRP3, Caspase-1, and IL-1β in HK-2 cells transfected with the miR-223-3p mimics or inhibitor were detected by Western blotting (e) and qRT-PCR (f). The data are presented as the of three independent experiments. ; , as determined by one-way ANOVA (c–f) and Pearson’s correlation test (b).

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