XIST Inhibition Attenuates Calcium Oxalate Nephrocalcinosis-Induced Renal Inflammation and Oxidative Injury via the miR-223/NLRP3 Pathway

<div>XIST inhibition alleviated CaOx crystal deposition and CaOx nephrocalcinosis-induced inflammatory and oxidative kidney injury via the XIST-miR-223-3p-NLRP3 ceRNA pathway. Polarization microscopy and Pizzolato staining were used to detect the crystal deposition in CaOx nephrocalcinosis model mice injected with rAAV-sh-XIST, antagomiR-223-3p, or both. (b) Kidney injuries were observed in CaOx nephrocalcinosis mice injected with rAAV-sh-XIST, antagomiR-223-3p, or both by PAS and TUNEL staining. The levels of NLRP3, Caspase-1, IL-1<i>β</i> (c), and SOD2 and NOX2 (d) in renal tissues were detected via IHC. 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602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,40.821,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,43.785,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,50.025,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>,</span> as determined by one-way ANOVA (a–d).</div>

Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: XIST Inhibition Attenuates Calcium Oxalate Nephrocalcinosis-Induced Renal Inflammation and Oxidative Injury via the miR-223/NLRP3 Pathway 