Exercise-Linked Irisin Prevents Mortality and Enhances Cognition in a Mice Model of Cerebral Ischemia by Regulating Klotho Expression

Figure 2

Swimming increased cerebral levels of irisin in mice. The expression of irisin in mice without MCAO after swimming for a different duration was detected by Western blot (a, b). The expression of irisin in the brain tissues of mice subjected to MCAO with or without swimming was detected by Western blot (c, d). Immunostaining showed that irisin was detected in the hippocampus of mice, and after swimming or exogenous irisin treatment, the expression of irisin in the hippocampus increased (e). Kaplan–Meier curves showed differences in survival rate among different groups (f), and the bodyweight of mice in the SW group and Ir group was higher than that in the MCAO group (g). Data are expressed as , per group, vs. S or Control, ^# vs. MCAO.

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