Exercise-Linked Irisin Prevents Mortality and Enhances Cognition in a Mice Model of Cerebral Ischemia by Regulating Klotho Expression

Figure 4

Irisin upregulated the expression of klotho and reduced oxidative stress in mice with MCAO. The experimental design was shown for the evaluation of the neuroprotective effects of irisin (a). Representative immunohistochemical images showed klotho expression in the hippocampus of mice in different groups (b). The expressions of klotho, FOXO3a, phosphorylated FOXO3a, and MnSOD were detected by Western blot (c–g). The extent of ROS production in the hippocampus of mice was determined by DHE staining (h). Data are expressed as , per group, vs. S, ^# vs. MCAO.

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