Exercise-Linked Irisin Prevents Mortality and Enhances Cognition in a Mice Model of Cerebral Ischemia by Regulating Klotho Expression

Figure 5

Irisin showed no neuroprotective effect on cognitive impairment in klotho^-/- mice. The experimental design was conducted for the evaluation of whether klotho mediated the neuroprotective effect of irisin (a). Kaplan–Meier curves showed differences in survival rate between WT mice and KO mice (b). The bodyweight of KO mice was lower than WT mice at 7 d and 14 d after MCAO (c). Mice in different groups were tested in the Morris water maze test (d–f). Mice in different groups were tested in an open field test (g, h), a novel object recognition task (i), and a fear conditioning test (j–m). Data are expressed as , per group, vs. WT.

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