Enhanced Expression of miR-34a Enhances Escherichia coli Lipopolysaccharide-Mediated Endometritis by Targeting LGR4 to Activate the NF-κB Pathway

Figure 2

The expression of LGR4 in cow uterus tissues. (a) H&E pathology of cow uterine tissues (HE, ), . (b) The mRNA levels of the inflammatory cytokines IL-1β, IL-6, TNF-α, and IL-10 detected by qRT-PCR to assess the extent of inflammation in uterine tissue. (c) The expression and location of LGR4 in uterine tissue by hematoxylin-eosin staining (H&E, ), . (d, e) The expression of LGR4 protein was determined by western blot, and its level was quantified by IPP 6.0. β-Actin was used as an internal control. (f) The LGR4 mRNA was analysed by qRT-PCR. GAPDH was used as a control. Control represents the control group, and Inf represents the naturally infected cow uterine tissue. Data are expressed as the of three independent experiments (). and compared with the control group (Student’s -test).

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