Enhanced Expression of miR-34a Enhances Escherichia coli Lipopolysaccharide-Mediated Endometritis by Targeting LGR4 to Activate the NF-κB Pathway

Figure 5

MiR-34a inversely correlates with the expression of LGR4. (a) The Venn diagram shows the predicted overlap of the microRNAs that target the cow and murine LGR4 3UTRs by miRcode, miRDB, TargetScan, and ENCORI. (b, c) The levels of the putative microRNAs, miR-34a, miR-34a, miR-34c, miR-218, and miR-302a were detected in BENDs treated with LPS (1.0 μg/ml) and cow uterus tissues by qRT-PCR. U6 snRNA was used as an endogenous control. (d) Cells were transfected with bovine miR-34a mimics, miR-34a inhibitor, or negative control (mimic NC or inhibitor NC) for 48 or 72 h, and then the miR-34a transcription levels were detected by qRT-PCR. (e, f) LGR4 mRNA and protein levels were determined by qRT-PCR and western blot, respectively. β-Actin was used as an internal control. Data are presented as the of three independent experiments (). and versus the control group (b–d); ^# versus the mimic NC group; versus the inhibitor NC group ((e and g) cells transfected with miR-34a mimic) (Student’s -test).

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