Enhanced Expression of miR-34a Enhances Escherichia coli Lipopolysaccharide-Mediated Endometritis by Targeting LGR4 to Activate the NF-κB Pathway

Figure 7

Overexpression of miR-34a promotes NF-κB p65 translocation into the nucleus in BENDs. (a, b) Cells were transfected with miR-34a mimics or miR-34a mimic NC for 24 h, followed by exposure to LPS for another 6 h. The relative protein expression of LGR4 and NF-κB p65 was measured by western blot. β-Actin was used as an internal control. (c) Immunofluorescence staining was performed to identify the expression of p-p65 (); . Blue spots represent cell nuclei, and red spots indicate p-p65 staining. (d) The intensity of p-p65. Values are given as the of three experiments (). and versus the control (b and d) or the mimic and LPS (d) group; ^# and ^## versus the LPS group.

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