Enhanced Expression of miR-34a Enhances Escherichia coli Lipopolysaccharide-Mediated Endometritis by Targeting LGR4 to Activate the NF-κB Pathway

Figure 8

Inhibition of miR-34a suppresses inflammation in BENDs. (a) Cells were transfected with miR-34a inhibitor or negative control (inhibitor NC) for 24 h and then stimulated with LPS (1.0 μg/ml) for 6 h. The relative protein expression of LGR4 and NF-κB p65 was measured by western blot. β-Actin was used as an internal control. (b) Grey values of LGR4 and NF-κB p65 protein were quantified by ImageJ software. (c, d) The secretion of IL-1β, IL-6, and TNF-α was detected by qRT-PCR. Values are given as the of three experiments (). and versus the inhibitor NC (cells transfected with miR-34a inhibitor NC) or mimic NC (cells transfected with miR-34a mimic NC) group; ^# versus the inhibitor group (b and d); ^@ compared with the LPS and inhibitor group (b).

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