Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

<div>Bavachin induces GSH depletion and lipid peroxidation accumulation in osteosarcoma (OS) cells. (a, b) Bavachin induces GSH depletion in a dose-related manner over 24 h. (c, d) Bavachin greatly induces ROS generation in OS cells over 24 h. (e, f) Bavachin increases MDA accumulation in a dose-related manner. (g, h) Western blotting analysis shows the expression of p-STAT3, STAT3, P53, SLC7A11, and GPX4 in OS cells treated with bavachin (10 <i>μ</i>M, 20 <i>μ</i>M, and 40 <i>μ</i>M) for 24 h. <sup>&amp;</sup><svg height="11.7782pt" id="M13" style="vertical-align:-3.42938pt" version="1.1" viewbox="-0.0498162 -8.34882 44.5261 11.7782" width="44.5261pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 -218 17 -231L13 -257L254 -247L259 -218L233 -216C183 -212 177 -202 189 -142L218 -1C238 -10 266 -12 283 -12C351 3 429 48 483 105C543 168 570 242 570 304ZM482 289C482 161 380 33 304 33C278 33 248 51 233 69L303 396C326 400 352 403 369 403C428 403 482 380 482 289Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.342,0)"><path d="M512 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells 