H2A.X Phosphorylation in Oxidative Stress and Risk Assessment in Plasma Medicine

Table 1

Overview of γH2A.X studies in plasma medicine. Both using jet plasmas and dielectric barrier discharge (DBD) as a source of cold physical plasma, γH2A.X was quantified in vitro in low and high malignant cancer cell lines as well as ex vivo in cold plasma-treated tissue.

	Plasma source	Reference
Low malignant cell line in vitro
HEK-293 (human embryonic kidney cells)	Soft jet plasma	Kaushik et al. [12]
MRC-5 (human lung fibroblasts)	Soft jet plasma	Kaushik et al. [12]
Human primary fibroblasts	Plasma needle	Lazović et al. [13]
TK6 cells (B lymphoblastoid cells)	kINPen	Bekeschus et al. [14]
HaCaT cells (human keratinocyte cells)	Jet plasma; kINPen	Gaur et al. [15]; Schmidt et al. [16]
MCF-10A (human breast epithelial cells)	DBD	Kalghatgi et al. [17]
Highly malignant line in vitro
A549 (lung adenocarcinoma)	Soft jet plasma	Kaushik et al. [12]
T98G (glioblastoma)	Soft jet plasma	Kaushik et al. [12]
U87MG (glioblastoma)	Jet plasma	Gjika et al. [18]
MSK QLL1, SCC1483, SCC15, and SCC25 (squamous cell carcinoma)	Jet plasma	Chang et al. [19]
SCC25 (squamous cell carcinoma)	Jet plasma	Han et al. [20]
Mel (melanoma); SK-MEL 28 (melanoma)	miniFlat-PlaSter; kINPen	Arndt et al. [21]; Sagwal et al. [22]
A549 (lung adenocarcinoma)	DBD	Karki et al. [23]
A2058 (melanoma); B16F10 (melanoma)	DBD	Sensing et al. [24]; Kim et al. [25]
HCT116 (colon carcinoma)	DBD	Judée et al. [26]; Plewa et al. [27]; Choi et al. [28]
Tissue ex vivo
Human skin	(mini)Flat-PlaSter	Isbary et al. [29]
Human oral mucosa	kINPen MED	Hasse et al. [30]
Porcine skin	DBD	Wu et al. [31]