Berberine Suppresses EMT in Liver and Gastric Carcinoma Cells through Combination with TGFβR Regulating TGF-β/Smad Pathway

Figure 7

Relation of TGF-β and Smad on EMT and proliferation in MGC803 cells. (a) Treated alone or cotreated SB (10 μM) and TGF-β (10 ng/ml) in 24 h; (10 μM) for 36 h in MGC803 cells by WB methods. (b) and (c) Treated alone or cotreated BBR (40 μM) and SB (10 μM) for 12 h and 36 h to detect relating proteins by WB method. (d) Treated with TGF-β (5, 10, 20, 40, 80, 160, and 320 ng/ml) in MGC803 cells to detect cell viability by MTT assays. (e) Treated with TGF-β (5, 10, 20, 40, and 80 ng/ml) in MGC803 cells to detect p-Smad2 by WB assay. (. , versus the control group, ^#, ^## versus the inhibitors group).

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